the Main Laboratory Biological Safety Equipment---Biological Safety Cabinets (Overview, Use, and the


1.Biological safety cabinet
The biological safety cabinets are negative pressure exhaust cabinets, used to protect the operator himself, laboratory environment and experimental materials when operating an infectious material,   so as to avoid exposing to the infection of aerosols and splatters in the process of operating.

2.The use of biological safety cabinet
(a) personal protection requirements: 1)must wear personal protective clothing and gloves (gloves should be set out in the scrubs) when using biological safety cabinets, 2)it is the best to wear masks and hats, when needing, take protective goggles or face mask. 3)Before work, the operator shall adjust the height of the stool to ensure that his face is above the opening.
(b) the principle of placing objects in biological safety cabinets:1) according to the direction from the clean area to the contaminated area. 2)Keep all items as far away from the front grille and close to the back of the operating table as possible as. 3)The contents of the waste bag, the tray for holding the waste straw and the larger volume of the filter bottle are placed on one side of the cabinet. 4)The front grille shall not be blocked by experimental records, instruments, equipment or other articles
(c) the biological safety cabinet operation: open the power button of the exhaust blower outside the biological safety cabinet  --open the operation ,window  slowly  , set a distance of 200mm , or  set value. General biological safety cabinet has window limit alarm --only need 10 minutes to begin the experiment operation -- after the operation ,pay attention to disinfection of biological safety cabinet.
(d) matters needing attention in the use of biosafety cabinets: 
1)The safety cabinet is only used when the work is normal.
2)Put two arms vertically into the front opening, wait in the biological safety cabinet for about a minute, so that the safe can be adjusted before it can be processed.
3)Wastes should be discarded in containers for biological safety cabinets to avoid rapid movement and frequent entry of the arm at the front opening so as not to disturb the airflow.
4)Avoid polluting items into clean areas.
5)When performing operations in a biological safety cabinet, no text work is permitted.
6)All the work of the cabinet should be carried out in the middle or rear face, and can be seen from the observation panel.
7)The front grille shall not be blocked notes, dropper, or other material, otherwise it will disrupt the airflow which may make material contamination, the personnel in the exposed state.
8)The cleaning and disinfection should be in every time after the completion of the work and the end of the day, the surface of the use of disinfectants to clean the appropriate safety cabinet.
9) The fan of the cabinet should run for 5 minutes before and after the completion of the work .

3.lab balance table
Lab balance table is designed to protect test specimens from dust or bacteria contamination by passing laminar air over the work area. On the lab balance table, no infectious material can be operated because its gas is discharged directly under positive pressure. Once the infectious sample is placed in the work area, laminar air will flow polluted air to the front of the operating personnel.
Air pressure
Gas emission
Protected object

Biological safety cabinet
discharge after cleaning
operators samples environment

Lab balance table
Positive pressure
Discharge directly

In addition to understanding the performance indexes of the safety cabinet, the best and fastest way to identify the most convenient safety cabinet safety performance is to understand whether you buy the bio safety cabinet won the following several major international safety cabinet certification: NSF49 (American biological safety cabinet standard - two biological safety cabinet), EN12469 (unified European bio safety cabinet etc.).